Scientists from the Nigerian Centre for Disease Control conducted a study utilizing metagenomic sequencing for pathogen surveillance.The devastation caused by Covid-19 pandemic led to the rapid development of new techniques, like metagenomics, and ignited a paradigm shift in how we identify, monitor, and respond to emerging pathogens.
Metagenomics
- Metagenomics is the study of microbes in their natural living environment, which involves the complex microbial communities in which they usually exist.
- The study examines the genomic composition of an entire organism, including each of the microbes that exist within it. It facilitates direct sequencing of patient samples, removing the need for prior knowledge of the infectious agent.
- For instance, a single gram of soil consists of 4000 to 5000 different species of microbes, while human intestines consist of 500 different types of bacteria.
- It enables us to understand the diversity, abundance, and interaction of microbes in any system.
- It is different from conventional sequencing methods, which requires culturing or isolating individual species before sequencing their genomes.
Genome Sequencing
- Genome sequencing is the process of determining the complete DNA sequence of an organism’s genome.
- DNA (deoxyribonucleic acid) is composed of a sequence of nucleotides, which are represented by the letters A, T, C, and G, corresponding to the four nucleotide bases: adenine, thymine, cytosine, and guanine.
- Genome sequencing involves identifying the order of these nucleotides along the DNA strands.
- The genome is the entire set of genetic material within an organism’s cells, and it contains all the information necessary for the growth, development, functioning, and reproduction of that organism.
- Genomic Surveillance and Covid-19 Pandemic: The global response to the Covid-19 pandemic prompted scientists to leverage genome sequencing technologies for surveillance purposes.
- The establishment of platforms like GISAID facilitated the submission and sharing of SARS-CoV-2 genome data, aiding high-throughput genome surveillance activities.
- High-throughput’ refers to sequencing techniques that can parse large quantities of DNA at the same time, including a whole genome at once.
- Potential: Genome sequencing’s potential extended to seasonal viruses like Zika and dengue, as well as diseases like lumpy skin disease in cattle and drug-resistant tuberculosis.